A generally applicable two-hybrid assay demonstrates that MHC class I heavy chains devoid of beta-2 microglobulin associate within and across allotypes, with implications for endocytosis and autoimmunity.
Micropatterned differentiation of human ESCs generates gastrulation cell types – germ layers, extraembryonic, and primordial germ cells with primate characteristics – that show conserved sorting behaviors when dissociated and reseeded as single-cell mixture.
The requirement for WNT signaling in mesendoderm differentiation is temporally separate from that of ACTIVIN signaling and acts to switch the output of ACTIVIN/SMAD2 from pluripotency maintenance to mesendoderm patterning.
A computational model, based on single-cell features like contractility and polarizability, quantitatively describes cellular dynamics from the single cell level up to small cohorts and confluent tissues.
A bioengineering approach identifies tissue morphology as an effective variable for controlling the inception of neural organoid morphogenesis via induction of a biomimetic, singular neural rosette tissue cytoarchitecture.