6,614 results found
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    A combined quantitative mass spectrometry and electron microscopy analysis of ribosomal 30S subunit assembly in E. coli

    Dipali G Sashital et al.
    Comprehensive structural and compositional characterization of the in vivo 30S ribosomal assembly landscape reveals parallel pathways for 3′-domain formation and a previously unknown role for the assembly factor RimP.
    1. Cell Biology
    2. Computational and Systems Biology

    CEM500K, a large-scale heterogeneous unlabeled cellular electron microscopy image dataset for deep learning

    Ryan Conrad, Kedar Narayan
    'Cellular Electron Microscopy 500,000 images' (CEM500K) is a highly heterogeneous, information-rich, non-redundant, unlabeled EM dataset curated to pre-train DL algorithms for better model generalization on EM segmentation tasks.
    1. Structural Biology and Molecular Biophysics

    PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy

    Sergey Gorelick et al.
    The PIE-scope resolves the limitations in correlative cryo-focused ion beam milling by adding robustness and ease of use to the workflow.
    1. Cell Biology

    A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics

    Etai Sapoznik et al.
    A novel single-objective light-sheet microscope with unparalleled spatiotemporal resolution enables imaging and optical manipulation of diverse biological specimens and processes.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    High-quality ultrastructural preservation using cryofixation for 3D electron microscopy of genetically labeled tissues

    Tin Ki Tsang et al.
    A broadly applicable method that faithfully preserves genetically labeled cellular structures for 3D electron microscopy (EM) and correlated light and electron microscopy (CLEM).
    1. Structural Biology and Molecular Biophysics

    2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy

    Melody G Campbell et al.
    Water molecules and amino-acid side chain rotamers have been resolved in the cryoEM structure of T20S proteasome at 2.8 angstroms.
    1. Cell Biology

    A robust method for particulate detection of a genetic tag for 3D electron microscopy

    James Rae et al.
    Development of a new system for gold particle detection of a genetic tag for electron microscopy.
    1. Microbiology and Infectious Disease

    Nanoscale organization of rotavirus replication machineries

    Yasel Garcés Suárez et al.
    Super-resolution microscopy reveals, at nanometric-scale, the highly organized protein structure of viroplasms, the viral factories used by rotavirus to replicate its genome and assemble new viral particles.
    1. Cell Biology
    2. Neuroscience

    Reconstruction of genetically identified neurons imaged by serial-section electron microscopy

    Maximilian Joesch et al.
    A new pipeline of electron microscopy techniques reduces the time required to visualize genetically targeted neurons and their connections by two orders of magnitude.
    1. Structural Biology and Molecular Biophysics

    Atomic model of the F420-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector

    Matteo Allegretti et al.
    The structure of a large enzyme complex involved in methanogenesis was determined at high resolution by electron cryo-microscopy.

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