Through the interaction between PCP signaling and N-cadherin, oriented cell division and cell rearrangement are coordinated to establish the appropriate tissue architecture critical for limb skeletal morphogenesis.
Building on previous work (Jinek et al., 2013), we report a simple and robust system to achieve high fidelity and high efficiency (30% of homologous recombination) genome engineering by homology-directed repair pathway in human cells using cell cycle synchronization and timed delivery of Cas9 ribonucleoprotein complexes.
Real-time monitoring of oxytocin-loaded vesicles and synaptic actin dynamics in zebrafish reveal that Slit3-Robo2-Cdc42 signalling maintains steady-state levels of mature oxytocin neuropeptide readily primed to be secreted upon physiological demand.
Formation of a phase-separated interface between homologous chromosomes during meiosis enables regulatory signals to spread in cis over long distances, illuminating the longstanding mystery of crossover interference.
Building on previous work (Conduit et al., 2014), and contrary to what was previously thought, it is shown that key centrosomal proteins are not recruited to centrosomes as part of large multi-protein assemblies.
A picorna-like virus promotes the accumulation of caspase-cleaved drosophila inhibitor of apoptosis 1 by inducing the degradation of NTAN1, a key N-end rule degradation pathway component, leading to apoptosis inhibition and viral replication enhancement.