Context-dependent optimization of Gli-binding site occupancy, in the presence of Hand2, is critical for modulating tissue-specific transcriptional output within tissues that lack an obvious Hedgehog morphogen gradient.
Advances in techniques for analysing single cells and tissues have inspired an international effort to create comprehensive reference maps of all human cells - the fundamental units of life - as a basis for both understanding human health and diagnosing, monitoring and treating disease.
Maternal positional information in the fly embryo can be read rapidly in spite of the gene-expression bottleneck and general examples of regulatory architectures that combine speed and accuracy are provided.
A mechanistic basis is provided for the regulative ability of the mammalian embryo offering a long-sought explanation for coordinating cell behaviors at the population level ensuring robustness in developmental outcome.
Propagation, speed and shapes of genetic waves of expression during development can be modeled by a simple interplay between two transcriptional modules (dynamic/static), which explains robustness and precision of patterning.
The gene Odd-paired is a late-acting regulator of zygotic gene expression, functioning coordinately with Zelda to influence chromatin accessibility and affecting genes expressed along both axes of Drosophila embryos.