Unique methodology was used to generate a large HDX-MS dataset to inform ligand-mediated activation of RORγ, an approach that can be extended to other nuclear receptors with unresolved ligand-dependent activities.
Hydrogen-deuterium exchange, electron microscopy, and vesicle reconstitution show how binding of the autophagy adaptor NDP52 to the FIP200 subunit of the ULK1 complex triggers membrane binding in autophagy.
Nuclear magnetic resonance analysis revealed the structural and dynamical changes in MgtE during the channel closure caused by the cooperative Mg2+-binding, which had remained undescribed only by a static crystal structure.
Translationally active polysomes from mammalian cells/tissues are successfully separated with rapidity, high efficiency and exceptional reproducibility by a SEC-based approach, an accessible alternative to the conventional sucrose density gradient analysis.
The formation of mutually exclusive coding and non-coding transcription units contributes to transcriptional interference and insulation at gene clusters and manages state-switching in response to environmental change.
Comprehensive mass spectrometry analysis of human plasma proteome reveals tissue leakage proteins, describes variability between individual plasma proteomes and demonstrates protein transfer across the placenta during pregnancy.
Perceived imminence of threat and resulting intensity of defensive responses during serial compound stimulus conditioning are determined by auditory stimulus salience, not cue sequence as recently reported.
Eukaryotic pathogens, like Cryptococcus deuterogattii, can use elevated mutation rates to more rapidly adapt to stresses, such as drug challenges, but at the cost of lower fitness in less stressful environments.