t-CyCIF can be used to collect spatially-encoded, multiparametric data from fixed and embedded research or clinical specimens making it possible to probe the organization of tumors and tissues at a single-cell level.

Imaging mass cytometry simultaneously lights up distinct immune cells in the brain.

By integrating multiplexed error-robust fluorescence in situ hybridization (MERFISH), deep learning, optical sectioning, and protocol optimization, thick-tissue 3D MERFISH allows volumetric single-cell transcriptome imaging of thick-tissue samples, broadening the scope of applications of spatial genomics.

A novel panel of homoFRET biosensors that detect kinase and second messenger activity, called FLAREs, enables multiparameter imaging of signaling activities within a single live cell with a quantitative and ratiometric readout.

A retracting gradient of the transcription factor Tailless spatiotemporally patterns the Drosophila tail region by modulating the intrinsic dynamics of a regulatory network involving the timer genes caudal, Dichaete, and odd-paired.

A high throughput, full lifespan microscopic analysis reveals that fission yeast does not age but has a finite replicative lifespan.

A multiplexed microfluidic device was developed to facilitate high-throughput screening of bacterial chemotaxis on a single chip and demonstrated across a range of microbes, chemostimulants, and chemical gradient conditions.

A multiplexed retrograde virus tracing method captures single-neuron projectome and transcriptome simultaneously.

An intersectional genetic vector approach allows the identification and characterization of novel hippocampal inhibitory neurons with both broad local connectivity within CA1 and long-range projections to several extrahippocampal areas.

Cancer stem cells that have undergone EMT are identified in the region surrounding metastatic human oral tumours in a retrospective cohort study, and these are predictive of metastatic outcome in an image-based machine learning approach.