Binding of a multivalent RNA-binding protein to mRNAs that are able to form pervasive RNA–RNA interactions induces formation of mesh-like condensates, whereas binding of mostly structured mRNAs induces sphere-like condensates.
Intersectin counterparts in yeast recruit WASP and WIP to endocytic sites to establish a robust multivalent SH3 domain-PRM interaction network which gives actin assembly onset a switch-like behavior in vivo.
Fibronectin can shift from a globular to fibrillated form when adsorbing to implant surfaces, and this shift makes it accessible to attachment by Staphylococcus epidermidis, which is the leading cause of implant-associated infections.
Diana M Mitrea, Jaclyn A Cika ... Richard W Kriwacki
Nucleolar protein localization involves the phase separation within the nucleolar matrix via three types of multivalent features: acidic tracts, nucleic acid binding domains and arginine-rich low complexity sequences.
Structural characterization of human RPA70N association with a series of DNA damage response proteins reveals versatile protein interaction mechanisms that help to recruit DNA repair proteins to the damage site quickly and efficiently.
Tyler S Harmon, Alex S Holehouse ... Rohit V Pappu
Computational modeling and theoretical analysis reveal how disordered linkers determine whether linear multivalent proteins undergo gelation with or without phase separation.
Robert McCoy Vernon, Paul Andrew Chong ... Julie Deborah Forman-Kay
Statistics on the frequencies of pi interactions in folded protein structures enable successful prediction of intrinsically disordered protein phase separation, with clear implications for a physical understanding of cellular organization.
Phase separation of two-component multivalent systems is suppressed at rational polymer stoichiometries, suggesting possible cellular strategies for regulating the formation and function of biomolecular condensates.
