Pup-On-target for Small molecule Target Identification Technology, POST-IT, is a revolutionary tool for accurately identifying protein targets of bioactive molecules in living cells and organisms, successfully identifying SEPHS2 and VPS37C as targets for dasatinib and hydroxychloroquine, respectively.

An analysis of the largest Salmonella Typhi genome collection to date (n=13,000) provides an updated overview of global genome diversity and antimicrobial resistance trends over time to inform public health action.

C. elegans nematodes can assess faithfully the threat that H₂O₂ poses by coupling the induction of their H₂O₂ defenses to the perception of high temperature—an inherent enhancer of the reactivity of H₂O₂.

Membrane homeostasis in bacteria relies on the controlled degradation of endogenous phospholipids by intracellular phospholipases A, however their structures and catalytic mechanism are still poorly understood.

A novel mechanism of intramembrane protease regulation involves a protein blocking the active site of the enzyme.

The β-barrel conformation of the KOW domain of the metamorphic protein RfaH is in equilibrium with a low-populated, predominately unstructured state exhibiting helical elements, suggesting that transiently structured elements in unfolded conformations might be a general scheme in fold-switching proteins.

Autosomal dominant IRF4 deficiency is the first genetic etiology of Whipple's disease, a very rare chronic condition following a rather common infection by Tropheryma whipplei.

Bacterial Type IV restriction-modification systems display remarkable, previously unnoticed diversity of complex gene and domain architectures, and are predicted to couple antiphage immunity with the abortive infection form of defense.

Topoisomerase VI selectively catalyzes strand passage of DNA molecules juxtaposed at close to a right angle, which explains the observed chiral-dependent activity and preferential DNA decatenation versus supercoil relaxation.

Human leukocyte antigen class I (HLA class I) polymorphisms dictate the degree of endo-lysosomal assembly, which can supplement endoplasmic reticulum assembly for constitutive HLA class I expression and increase the efficiency of cross-presentation.