879 results found
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    The Rqc2/Tae2 subunit of the ribosome-associated quality control (RQC) complex marks ribosome-stalled nascent polypeptide chains for aggregation

    Ryo Yonashiro et al.
    Rqc2-mediated modification of ribosome-stalled nascent chains with "CAT tails" promotes formation of insoluble aggregates suggesting that proteins can become specifically marked for aggregation.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    The force-sensing peptide VemP employs extreme compaction and secondary structure formation to induce ribosomal stalling

    Ting Su et al.
    The structure of the VemP-stalled ribosome reveals a helix-double turn-helix conformation of the nascent chain within the ribosomal tunnel, illustrating how secondary structure formation directly at the peptidyltransferase center of the ribosome can induce translational arrest.
    1. Biochemistry and Chemical Biology

    Gradual compaction of the nascent peptide during cotranslational folding on the ribosome

    Marija Liutkute et al.
    HemK NTD cotranslational folding starts within the ribosome exit tunnel upon N-terminal helix synthesis and proceeds sequentially through a series of intermediates becoming less dynamic as the nascent chain grows.
    1. Biochemistry and Chemical Biology

    Fine interaction profiling of VemP and mechanisms responsible for its translocation-coupled arrest-cancelation

    Ryoji Miyazaki et al.
    Time-resolved and site-directed in vivo photo-crosslinking analysis of VemP allows identification of both cis- and trans-elements required for its regulated arrest-cancelation.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Elongation inhibitors do not prevent the release of puromycylated nascent polypeptide chains from ribosomes

    Benjamin D Hobson et al.
    Although puromycin staining is often used to examine subcellular translation, puromycin-labeled proteins are rapidly released from ribosomes even in the presence of elongation inhibitors, which may confound translation site localization.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Real-time observation of signal recognition particle binding to actively translating ribosomes

    Thomas R Noriega et al.
    The signal recognition particle only stably engages translating ribosome nascent-chain complexes exposing a functional signal sequence.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Structural and mutational analysis of the ribosome-arresting human XBP1u

    Vivekanandan Shanmuganathan et al.
    Two integrated approaches shed light on how XBP1 arrest peptide induces intermediate level of translational pausing and identify hotspot positions to make it stronger.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Structures of the scanning and engaged states of the mammalian SRP-ribosome complex

    Rebecca M Voorhees, Ramanujan S Hegde
    Structures of the signal recognition particle before and after it captures a transmembrane domain suggest how it chooses, engages, and shields its clients during membrane protein targeting to the endoplasmic reticulum.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Residue-by-residue analysis of cotranslational membrane protein integration in vivo

    Felix Nicolaus et al.
    The cotranslational membrane integration of three multispanning Escherichia coli inner membrane proteins is followed using force profile analysis, uncovering unexpected complexities in the membrane integration process.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Dynamics of ribosomes and release factors during translation termination in E. coli

    Sarah Adio et al.
    Translation termination is a stochastic process that utilizes loosely coupled motions of its players to complete protein synthesis and release the newly synthesized nascent chain toward its cellular destination.

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