A screen of visual-experience induced changes in newly-synthesized proteins in Xenopus optic tectum identifies unexpected candidate plasticity proteins.

Newly-synthesized protein can be labeled with cellular specificity in Drosophila brain using the puromycin analog PhAc-OPP coupled to expression of the unblocking enzyme PGA in a tissue or cell type of interest.

Metabolic labelling and quantitative proteomics reveal the temporal evolution of cellular and neuronal responses to global activity manipulations.

Analysis of protein interfaces suggests cotranslational assembly can be an adaptive process, likely serving to minimise non-specific interactions with other proteins in the cell.

In neurons, inhibition of the proteasome results in feedback inhibition of protein synthesis, mediated by heme-regulated kinase 1, which is optimized to act as both a sensor and an effector.

Dynamic SILAC labeling in combination with mass spectrometry revealed substantial regulation of protein synthesis, degradation, turnover, and abundance during homeostatic scaling in neurons.

METTL18 methylates histidine 245 of ribosomal protein RPL3, retards ribosome traversal at tyrosine codons, and thus ensures nascent protein folding for proteostasis maintenance.

Genetic and biochemical approaches reveal that mammalian cells possess a novel DDI2 and transcription-independent pathway for the rapid recovery of proteasome activity after clinically relevant pulse treatment with proteasome inhibitors.

Quantitative protein and ribosome 'accounting' in T cells highlights a gap in our understanding of how activated T cells sufficiently replicate their proteome during rapid cell division driven by infection.

Tom70 connects the mitochondrial import to the nuclear transcriptional activity of mitochondrial proteins and controls mitochondrial biogenesis defects during aging.