Structural studies reveal the mechanism by which a HECT E3 ubiquitin ligase carries out E2-to-E3-to-substrate ubiquitin transfer and prioritizes target lysines for ubiquitination.

Random sequence RNA pools display an innate capacity for ligation and recombination, enabling them to “bootstrap” themselves towards higher compositional, informational and structural complexity.

Legionella effector RavZ evades host autophagy by extracting LC3-PE from the membrane before deconjugation.

Snap-freezing of brain tissue reveals its true structure-showing that cells are less squashed together, and the connections between neurons are less enclosed than previously thought.

Cryo preservation of brain tissue reveals dendritic spines with thinner necks compared to those prepared with chemical fixation.

A multidimensional chemical mapping strategy enables confident determination of the structures of non-coding RNAs at 1-nm resolution, including previously intractable riboswitch and human regulon states.

A broadly applicable method that faithfully preserves genetically labeled cellular structures for 3D electron microscopy (EM) and correlated light and electron microscopy (CLEM).

The first crystal structure of an active plant asparaginyl endopeptidase reveals a tetrahedral intermediate state in its active site, which may help to explain why these enzymes have been independently recruited to perform peptide macrocyclization.

A stable tetrameric nucleosome occupies the central segment of each ∼120-bp budding yeast centromere in two rotational phases of both reflectional orientations in vivo.

By harnessing antibody fragments conjugated to small gold nanoparticles together with high pressure freezing, FIB/SEM milling and cryo-electron tomography, AMPA receptors were identified from mouse brain tissue.