We discuss the methods available to understand lncRNA function in vivo, and highlight important considerations that should be taken into account when designing such experiments.
Functional and mechanistic analysis of p53-regulated lncRNA PINCR and its interacting RNA-binding protein Matrin 3 uncovers context-dependent regulation of specific p53 target genes during DNA damage.
Interaction with PUMILIO is essential for maintenance of genomic stability by the cytoplasmic long noncoding RNA NORAD, whereas binding to RBMX is dispensable for this function.
Single-cell RNA sequencing of Drosophila hemocytes in blood distinguishes various states within known blood cell types and implicates a novel role for fibroblast growth factor receptor signaling in inter-hemocyte crosstalk.
Recent examples demonstrate that already the transcriptional activity at lncRNA genes does have an important function on gene regulation, while the produced non-coding RNA might be dispensable.
Genes implicated in the control of mammalian puberty function as components of a molecular clock that determines the timing of sexual differentiation in the C. elegans nervous system.
RNA Polymerase II transcriptional termination of a nuclear long non-coding RNA is required to maintain the mitochondrial genome and hence promotes budding yeast growth.
