Morphological profiling allows accurate identification of cell types in dense iPSC-derived cultures, allowing its use for quality control and differentiation monitoring.

In developing neural cell cultures, information flows lock-in early and nodes undertake specialised computational roles.

Flow cytometric isolation and fate mapping shows that neurosphere-initiating cells are highly mitotically active and persist only transiently in vivo, and are distinct from quiescent, long-lived neural stem cells.

Genetic and protein analyses in vitro and in vivo identifies Id4 as a novel regulator of stem cell quiescence in the adult hippocampus.

Automated liquid handling, whole mount staining, and clearing allow unbiased 3D quantitation of cell markers in human neural organoids with diameters of up to 1 mm at the single-cell level.

Interaction of human embryonic stem cells (hESC) with human amniotic epithelial cells (hAEC) confers hESC a pluripotent potential that resembles the anteriorized epiblast, which is predisposed to form the neural ectoderm.

A dynamic SILAC analysis reveals that neuronal proteome turnover is influenced by the cell type as well as the extracellular environment.

A novel 2D-to-3D human brain organoid protocol reveals the pivotal role of FGF8 signaling in driving regional patterning and cellular diversity, while also modulating genes crucial for both normal and pathological neural development.

Akt activation is required for neural crest differentiation through regulation of Sox10 activity.

Stem cell derived ventral-spinal cord excitatory neurons self-assemble into a rhythmically bursting neural network whose speed and intercellular coordination are both instructively modulated by cell-type specific interactions with inhibitory neurons.