A single-cell transcriptomic resource of the early chick epiblast from mid-gastrulation through neurulation highlighting emergence of the neural plate border territory, with emphasis on neural crest specification.

The vertebrate neural plate border is comprised of precursors that coexpress multiple lineage markers and small changes in their levels can bias border cell fate.

Neural plate border cells co-express neural, neural crest, and placodal transcriptional programs before cell fate decisions occur and are transcriptionally heterogeneous along the antero-posterior and medial-lateral axis.

Chromatin remodeler Hmga1 has two separable functions in neural crest development, first neural crest specification at the neural plate border and later in Wnt-dependent emigration from the neural tube.

BMP-dependent retinoic acid signaling is responsible for the separation of central from peripheral lineages during neural development.

Akt activation is required for neural crest differentiation through regulation of Sox10 activity.

Automated liquid handling, whole mount staining, and clearing allow unbiased 3D quantitation of cell markers in human neural organoids with diameters of up to 1 mm at the single-cell level.

A new cell type at the border of the fourth ventricle is the exclusive source of roof plate.

In addition to its role in regulating proliferation and cell death, the PTEN tumor suppressor regulates epithelial morphogenesis through the PDK1 kinase.

A comprehensive resource based on analysis of the responses of embryonic ectoderm cells to signals from the 'organizer' (the node) including transcriptional responses and epigenetic changes with fine temporal dynamics, predicted regulatory interactions, and conservation among vertebrates.