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    1. Neuroscience

    Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors

    Austin R Graves, Richard H Roth ... Richard L Huganir
    A knockin mouse line was made to label endogenous AMPA receptors, enabling direct observation of synaptic plasticity in behaving animals with single-synapse resolution across the entire brain.
    1. Structural Biology and Molecular Biophysics

    Atomistic Tuning of the GeoCas9 Recognition Lobe Modulates Allosteric Motions and Guide RNA Interactions

    Helen B Belato, Alexa L Knight ... George P Lisi
    Not revised
    Reviewed Preprint v1
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    • Incomplete
    1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    Dual expression of Atoh1 and Ikzf2 promotes transformation of adult cochlear supporting cells into outer hair cells

    Suhong Sun, Shuting Li ... Zhiyong Liu
    Adult cochlear supporting cells (SCs) are plastic and respond to ectopic Ikzf2 and Atoh1, and hair cell damage by up-regulating HC and down-regulating their endogenous SC genes.
    1. Chromosomes and Gene Expression
    2. Immunology and Inflammation

    SRSF6 balances mitochondrial-driven innate immune outcomes through alternative splicing of BAX

    Allison R Wagner, Chi G Weindel ... Kristin L Patrick
    The splicing factor SRSF6 helps regulate antiviral immunity and mitochondrial membrane integrity by balancing the abundance of two alternatively spliced isoforms of the apoptotic protein BAX.
    1. Microbiology and Infectious Disease
    2. Structural Biology and Molecular Biophysics

    A pH-dependent cluster of charges in a conserved cryptic pocket on flaviviral envelopes

    Lorena Zuzic, Jan K Marzinek ... Peter J Bond
    Benzene mapping simulations of envelope protein rafts from six different flaviviruses reveal a conserved cryptic site whose cluster of ionisable residues is likely responsible for orchestrating pH-dependent conformational changes during fusion, thereby representing an attractive target for antiviral development.
    1. Cancer Biology
    2. Genetics and Genomics

    Single-cell multiomics analysis of chronic myeloid leukemia links cellular heterogeneity to therapy response

    Rebecca Warfvinge, Linda Geironson Ulfsson ... Göran Karlsson
    Single-cell analysis of CML patients bone marrow at diagnosis reveals coexistence of CD26-CD35+ healthy stem cells and CD26+CD35- CML stem cells, and how the ratio between these impacts TKI response.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Translational control of one-carbon metabolism underpins ribosomal protein phenotypes in cell division and longevity

    Nairita Maitra, Chong He ... Michael Polymenis
    Extensive molecular profiling shows how loss of highly similar, paralogous ribosomal proteins lead to distinct phenotypic outputs, through translational control of specific mRNAs.
    1. Microbiology and Infectious Disease

    Magnesium modulates phospholipid metabolism to promote bacterial phenotypic resistance to antibiotics

    Hui Li, Jun Yang ... Bo Peng
    Dysregulated unsaturated fatty acids and saturated fatty acids contribute to bacterial phenotypic resistance to antibiotics.
    1. Ecology

    Experimental evidence that chronic outgroup conflict reduces reproductive success in a cooperatively breeding fish

    Ines Braga Goncalves, Andrew N Radford
    Outgroup conflict can negatively impact general reproductive behaviour, egg investment, and parental care, resulting in decreased reproductive output even in the absence of physical confrontations.
    1. Ecology
    2. Evolutionary Biology

    The African ape-like foot of Ardipithecus ramidus and its implications for the origin of bipedalism

    Thomas Cody Prang
    The most recent common ancestor of humans, chimpanzees, and bonobos possessed a foot adapted to terrestrial quadrupedalism and climbing.