A survey of oxygen-dependent enzymes suggests new candidates for oxygen sensors, expanding potential mechanisms underlying hypoxia-related adaptations or diseases in humans.
A genetic screen identifies a lipid pathway which impacts the endoplasmic reticulum unfolded protein response and highlights a mechanism through which lipid disequilibrium might facilitate the progression of proteopathic diseases.
Abderrahman Hachani, Stefano G Giulieri ... Timothy P Stinear
Staphylococcus aureus switches from innocuous coloniser to invasive human pathogen, the bacterial population can accumulate pathoadaptive mutations that reduce bacterial toxicity whilst allowing persistence within infected cells, potentially leading to severe human infections.
Stefanny Villalobos-Cantor, Ruth M Barrett ... Ian Martin
Newly-synthesized protein can be labeled with cellular specificity in Drosophila brain using the puromycin analog PhAc-OPP coupled to expression of the unblocking enzyme PGA in a tissue or cell type of interest.
Microbial cells optimally structure their proteomes in order to mutually maximize metabolism and translation, as established by an extensive comparison between data and a low-dimensional model of cellular physiology.
Under nitrogen limitation, Salmonella and Escherichia coli express a messenger RNA to translate glutamine synthetase, and concomitantly the messenger RNA produces a small RNA from its 3′ end to inhibit the translation of 2-oxoglutarate dehydrogenase of the TCA cycle.
Olga T Schubert, Joshua S Bloom ... Leonid Kruglyak
A CRISPR/Cas9 base editor screen allows probing interactions between large numbers of known mutations and the abundance of specific proteins to study the protein regulatory network in yeast.
Sudipta Mondal, Priyadarshan Kinatukara ... Rajan Sankaranarayanan
DIP2 is a conserved protein across fungi and animals that regulates specific diacylglycerol pools by diverting them to storage lipid biosynthesis to enable cellular homeostasis and adaptations.
Robert A Crawford, Mark P Ashe ... Graham D Pavitt
Using mass spectrometry to study protein composition of translating ribosomes under unstressed or stress conditions, aspartate aminotransferase 2 (Aat2) is identified as a metabolic enzyme with a moonlighting function in the yeast integrated stress-response pathway.
METTL18 methylates histidine 245 of ribosomal protein RPL3, retards ribosome traversal at tyrosine codons, and thus ensures nascent protein folding for proteostasis maintenance.
