First structural view of a substrate-bound PIKK (PI3K-related-kinase) by cryo-EM explains phosphorylation specificity toward a short amino-acid motif shared across the PIKK family and rationalizes PIKK auto-inhibition by regulatory domain.
The myopathic transcription factor DUX4 induces discordant dysregulation of transcript and protein levels, demonstrating a key role for post-transcriptional gene regulation in facioscapulohumeral muscular dystrophy.
RNA-binding protein SRSF3 mediates critical changes in RNA processing of pluripotency genes, which reveals functional consequences of regulated RNA processing during stem cell self-renewal and early development.
Nucleotide resolution mapping of ribosome-bound RNA decay fragments in yeast cells expressing ATPase-deficient UPF1 reveals 3' UTR bound ribosomes and a role for UPF1 in post-termination ribosome recycling.
Fundamental details of the rate and molecular spectrum of transcript errors were revealed in four bacterial species, providing novel insights into transcriptional fidelity and RNA quality-control in prokaryotes.