The new whale shark genome assembly represents the best gapless chondrichthyan genome assembly yet, and comparative genomic analyses provide insights into the evolution of vertebrate genome origins, immunity, and gigantism.

Replication origins are established throughout the genome with the exception of transcribed genes, and the local chromatin composition likely modulates the density of ORC and MCM as well as origin activation.

Two novel subsets of microglia identified by their unique autofluorescence profiles differ in their subcellular organization, proteomic signatures and in their response to aging and lysosomal dysfunction.

In depth characterization of gene expression in the mouse hypothalamus will facilitate understanding of the molecular pathways that affect metabolic traits and discovers new genes associated with these pathways.

Single-molecule analysis of SARS-CoV-2 RNA identifies significant heterogeneity in cellular viral RNA levels and highlights slower replication kinetics for the Alpha variant compared to the Victoria strain.

One hundred one high-quality drosophilid genomes are released, along with low-cost assembly workflows, as an open community resource for studying genetics, ecology, and evolution in this important model system.

A deep neural network trained on semi-synthetic data learns to quickly track and identify neurons in Caenorhabditis elegans.

The viscous hydraulics of contracting endoplasmic reticulum networks challenge common solute transport theories, suggesting the contraction of peripheral sheets as a plausible driving mechanism.

The crystal structure of the trans-activation response region (TAR) bound to HIV-1 Tat and an elongation factor, together with HDX, SHAPE, SAXS, and integrative modeling, shows how TAR binds this complex in two ways.

Several new magneto-mechanical and magneto-thermal mechanisms of ion channel activation in magnetogenetics are proposed that may explain some of the mysteries that challenge current understanding of the magnetogenetics experiments.