Micromanipulation experiments demonstrate that HP1α strengthens interphase and mitotic chromosome mechanics and maintains nuclear shape by chromatin crosslinking, separate from histone methylation.

Nuclear reorganization and stiffening accompanies mesenchymal stem cell differentiation, resulting in increased sensitivity to mechanical perturbation.

Animal RanBP1 nuclear export and cargo dissociation mechanisms are surprisingly different from yeast, due to mutations of critical residues, leading to greater nuclear transport efficiency and higher energy cost.

NMR spectroscopy has been used to explain a central unresolved issue of nuclear transport, namely how it can be both fast and specific.

The Tsr2 carrier protein connects the nuclear import machinery with the ribosome assembly pathway.

ESCRT-driven mechanisms that sense and seal holes in the nuclear membranes directly monitor the nuclear transport system and the exposure of the inner nuclear membrane.

Advanced microscopy techniques reveal unique biological features that distinguish androgen receptor variant 7 (AR-V7) from the canonical AR, including high intranuclear mobility and rapid nuclear import that occurs independently of importin-α/β.

Structural and biochemical studies of Kap123 revealed the mechanisms by which Kap123 recognizes H3- and H4-NLSs through two lysine-binding pockets and by which evolutionarily conserved diacetylation of H4-NLS facilitates Kap123-H3-NLS mediated nuclear translocation.

Microtubule nucleation from the nuclear envelope in fission yeast involves repurposing of nuclear export proteins for a non-export-related function, docking cytoplasmic proteins at nuclear pore complexes.

Confronting different models of chromatin accessibility with temporally resolved transcription profiles favors a scenario where transcription factors actively, rather than passively, drive chromatin from the inaccessible to the accessible state.