295 results found
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Autocatalytic microtubule nucleation determines the size and mass of Xenopus laevis egg extract spindles

    Franziska Decker et al.
    Quantitative microscopy and theory show that the size of Xenopus laevis egg extract spindles is controlled by a spatially-regulated autocatalytic growth mechanism driven by microtubule-stimulated microtubule nucleation.
    1. Cell Biology

    The microtubule polymerase Stu2 promotes oligomerization of the γ-TuSC for cytoplasmic microtubule nucleation

    Judith Gunzelmann et al.
    Biochemical and genetic approaches show that the XMAP215 homolog Stu2 directly interacts with the small gamma-tubulin complex and its recruitment factor Spc72 to instigate functions in cytoplasmic microtubule organization.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Cell-cycle dependent phosphorylation of yeast pericentrin regulates γ-TuSC-mediated microtubule nucleation

    Tien-chen Lin et al.
    Phosphorylation of Spc110 N-terminal domain encompassing conserved motifs and its interaction with conserved GCP3 N-terminal domain regulate the oligomerization of gamma-tubulin small complexes (γ-TuSCs).
    1. Cell Biology

    A mitochondria-anchored isoform of the actin-nucleating spire protein regulates mitochondrial division

    Uri Manor et al.
    An actin nucleating protein, Spire1C, localizes to the mitochondrial outer membrane and interacts with the endoplasmic reticulum-anchored formin protein INF2 to drive mitochondrial fission.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Structural insight into TPX2-stimulated microtubule assembly

    Rui Zhang et al.
    A combination of cryo-electron microscopy of TPX2 bound to microtubules and in vitro reconstitution experiments reveals a novel microtubule interaction mode that explains how TPX2 promotes microtubule nucleation and stabilization.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Three-color single molecule imaging shows WASP detachment from Arp2/3 complex triggers actin filament branch formation

    Benjamin A Smith et al.
    New insights into the mechanism responsible for the nucleation of branches on networks of actin filaments shed light on a process that is essential for eukaryotic cell motility and endocytosis.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    An engineered monomer binding-protein for α-synuclein efficiently inhibits the proliferation of amyloid fibrils

    Emil Dandanell Agerschou et al.
    The high affinity α-synuclein-monomer binder AS69 converts into a strong sub-stoichiometric inhibitor of nucleation processes upon formation of the AS69-α-synuclein complex, achieving reduced aggregation in vitro and in vivo.
    1. Cell Biology
    2. Physics of Living Systems

    Cortical microtubule nucleation can organise the cytoskeleton of Drosophila oocytes to define the anteroposterior axis

    Philipp Khuc Trong et al.
    A three-dimensional description of the cytoskeletal arrangement, cytoplasmic flows, and cargo transport in stage 9 Drosophila oocytes accurately reproduces mRNA localizations in wild-type and mutant oocytes.
    1. Cell Biology
    2. Physics of Living Systems

    Spatiotemporal organization of branched microtubule networks

    Akanksha Thawani et al.
    A sequential reaction pathway involving TPX2, augmin and γ-TuRC governs the assembly and architecture of branched microtubule networks.
    1. Physics of Living Systems
    2. Cell Biology

    Physical basis of large microtubule aster growth

    Keisuke Ishihara et al.
    Autocatalytic growth of a microtubule polymer network allows extremely large egg cells to self-organize and divide rapidly.

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