428 results found
    1. Computational and Systems Biology
    2. Genetics and Genomics

    The genetic landscape for amyloid beta fibril nucleation accurately discriminates familial Alzheimer’s disease mutations

    Mireia Seuma et al.
    A massively parallel analysis of the effects of mutations on amyloid beta nucleation provide the first comprehensive atlas of how mutations alter the formation of amyloid fibrils.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Autocatalytic microtubule nucleation determines the size and mass of Xenopus laevis egg extract spindles

    Franziska Decker et al.
    Quantitative microscopy and theory show that the size of Xenopus laevis egg extract spindles is controlled by a spatially-regulated autocatalytic growth mechanism driven by microtubule-stimulated microtubule nucleation.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    An engineered monomer binding-protein for α-synuclein efficiently inhibits the proliferation of amyloid fibrils

    Emil Dandanell Agerschou et al.
    The high affinity α-synuclein-monomer binder AS69 converts into a strong sub-stoichiometric inhibitor of nucleation processes upon formation of the AS69-α-synuclein complex, achieving reduced aggregation in vitro and in vivo.
    1. Cell Biology
    2. Physics of Living Systems

    The transition state and regulation of γ-TuRC-mediated microtubule nucleation revealed by single molecule microscopy

    Akanksha Thawani et al.
    Four αβ-tubulin dimers associate laterally on γ-TuRC to define the rate-limiting transition state for microtubule nucleation.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Biochemical reconstitution of branching microtubule nucleation

    Raymundo Alfaro-Aco et al.
    The protein TPX2 and the protein complex augmin together recruit the microtubule nucleator γ-TuRC to a pre-existing microtubule where they initiate branching microtubule nucleation.
    1. Cell Biology

    The microtubule polymerase Stu2 promotes oligomerization of the γ-TuSC for cytoplasmic microtubule nucleation

    Judith Gunzelmann et al.
    Biochemical and genetic approaches show that the XMAP215 homolog Stu2 directly interacts with the small gamma-tubulin complex and its recruitment factor Spc72 to instigate functions in cytoplasmic microtubule organization.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Cell-cycle dependent phosphorylation of yeast pericentrin regulates γ-TuSC-mediated microtubule nucleation

    Tien-chen Lin et al.
    Phosphorylation of Spc110 N-terminal domain encompassing conserved motifs and its interaction with conserved GCP3 N-terminal domain regulate the oligomerization of gamma-tubulin small complexes (γ-TuSCs).
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    CM1-driven assembly and activation of yeast γ-tubulin small complex underlies microtubule nucleation

    Axel F Brilot et al.
    Cryo-EM structures reveal that the conserved CM1 motif drives γTuRC assembly by bridging adjacent γTuSC.
    1. Cell Biology

    In vitro reconstitution of branching microtubule nucleation

    Ammarah Tariq et al.
    Purification of two conserved protein complexes, the γ-TuRC and Augmin, using a simple affinity technique, demonstrates that they are necessary and sufficient for the essential phenomenon of branching microtubule nucleation.
    1. Cell Biology

    A mitochondria-anchored isoform of the actin-nucleating spire protein regulates mitochondrial division

    Uri Manor et al.
    An actin nucleating protein, Spire1C, localizes to the mitochondrial outer membrane and interacts with the endoplasmic reticulum-anchored formin protein INF2 to drive mitochondrial fission.

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