Heterochromatin is established at nucleation sites associated with open chromatin and RNA transcripts and matures into stable domains if they are found near transposable elements targeted by maternally deposited piRNAs.
A combination of cryo-electron microscopy of TPX2 bound to microtubules and in vitro reconstitution experiments reveals a novel microtubule interaction mode that explains how TPX2 promotes microtubule nucleation and stabilization.
Single-molecule TIRF microscopy, biochemical assays and mathematical modeling demonstrate how actin-branch nucleation by the Arp2/3 complex is coordinately regulated by two biochemically distinct activators.
A three-dimensional description of the cytoskeletal arrangement, cytoplasmic flows, and cargo transport in stage 9 Drosophila oocytes accurately reproduces mRNA localizations in wild-type and mutant oocytes.
Structural rearrangements of meiotic spindles during the transition from metaphase to anaphase can be controlled by local changes of microtubule dynamics, such as nucleation and/or turnover, and that katanin promotes microtubule turnover by severing microtubules near the chromosomes.