Unlike Chd1, ISWI remodelers preferentially shift asymmetric nucleosomes toward the side possessing a wild type acidic patch, regardless of DNA lengths flanking the nucleosome.
Regulation of the INO80 chromatin remodelling complex differs from those identified in other chromatin remodellers and involves a C-terminal domain of the Ino80 subunit.
Oriented hexasomes can be generated using the Widom 601 positioning sequence, which enables straightforward production of nucleosomes with asymmetrically modified H2A/H2B dimers.
Single molecule DNA unzipping reveals a novel model for the regulation of transcription factors and nucleosome positioning via nucleosome remodeling in yeast.
Histone variant H2A.Z is deposited near transcription start sites by the chromatin remodeler SWR1 and seems to be removed by RNA polymerase II at an early stage of transcription elongation.
Specific elements of the canonical nucleosome are recognized by the multi-component SWR1 chromatin remodeler for ATP-dependent replacement of H2A-H2B dimers with H2A.Z-H2B.