A one-photon three-dimensional light sculpture technique has been developed and demonstrated for high spatio-temporal resolution photostimulation and imaging of dozens of neurons in the intact mouse brain in vivo.
Andrea Giovannucci, Johannes Friedrich ... Eftychios A Pnevmatikakis
CaImAn is an open-software package that equips the neuroscience community with a set of turnkey, fast and scalable solutions to pre-processing problems arising in single cell calcium imaging data analysis.
Julianne M Troiano, Federico Perozeni ... Gabriela S Schlau-Cohen
Light-harvesting complex stress-related is a protein from photosynthetic green algae that prevents damage from sunlight via two distinct conformational processes, which protect against different timescales of solar fluctuations.
Elin Claesson, Weixiao Yuan Wahlgren ... Sebastian Westenhoff
Time-resolved crystallography provides insight into the photochemical reactions in photoreceptor proteins, explaining the earliest steps of how plants, fungi and bacteria sense red light.
Widefield fluorescence from the brain arises from greater depth and area than typically appreciated and is usually a weighted average across cortical columns and often more than one cortical area.
The overall energy conversion efficiency is calculated for a bacterial vesicle that harvests solar energy for ATP production on the basis of an atomic-detail structural model.
New experiments and theory reveal how the ability to see image details depends upon photoreceptor function and eye movements, and how fruit flies (Drosophila) see spatial details beyond the optical limit of their compound eyes.
A transport mechanism is uncovered in the major drug-efflux system in E. coli involving two remote alternating-access conformational cycles, which could provide the basis for the development of allosteric inhibitors against multidrug resistance.
