The combined use of optical trapping and single-molecule FRET permits the study of riboswitch structure formation and conformational dynamics at the same time.
Cardiac myosin converts energy from ATP into mechanical work by transitioning from a short-lived force-bearing state, to a post working stroke state before the release of inorganic phosphate.
A general framework for gating reveals that inter-head tension is not essential for coordinating kinesin stepping, and that neck-linker length is tuned to enhance processivity and velocity.
Unlike other cytoskeletal motors that produce torque in a specific direction, cytoplasmic dynein generates torque in either direction, resulting in bidirectional helical motility along microtubules.
The protofilaments that curl outward from a disassembling microtubule tip carry a large amount of strain energy and they can drive movement with an efficiency similar to conventional motor proteins.
The Janelia Research Campus opened its doors 12 years ago: we describe the philosophy underpinning Janelia, the lessons learned, and plans for the future.
