Whole endosome recording shows that chloride interacts with vesicular glutamate transporters as both allosteric activator and permeant ion, and although the mode of permeation differs, chloride and glutamate use a related conduction pathway.
Carolina Alvadia, Novandy K Lim ... Cristina Paulino
While activated by a common mechanism, both functions in TMEM16F - lipid scrambling and ion conduction - are likely mediated by alternate protein conformations that are at equilibrium in the ligand-bound state.
TMEM206 proteins are identified as constituting the pore of the widely expressed acid-activated chloride channel PAORAC/ASOR, which is important in acid toxicity.
GluK1-1 splice variant alters the gating properties of kainate receptors, providing crucial insights into receptor modulation by auxiliary proteins and advancing the understanding of synaptic transmission.
Aaron L Randolph, Younes Mokrab ... Ian Scott Ramsey
Electrophysiological and computational techniques provide a structural basis for understanding the conformational changes that are required to activate Hv1 proton channels.
Viktor V Feketa, Yury A Nikolaev ... Elena O Gracheva
CNGA3 is a cold-potentiated ion channel that confers cold sensitivity to mouse hypothalamic neurons, whereas CNGA3 from hibernating ground squirrels is cold-insensitive.
Single-particle cryo-EM and electrophysiology studies of the chloride channel TMEM16A reveals the structural basis for anion conduction and uncover its relationship to lipid scramblases of the same family.
Combined simulations and electrophysiological experiments show that the CLC channels and exchangers form physically distinct and evolutionarily conserved pathways through which Cl- and H+ ions move when crossing biological membranes.
