A simple and effective method facilitates the study of in vivo transcriptional dynamics using transcriptional enhancers and destabilized fluorescent protein, which is suitable for both live imaging and fixed studies.
Distinct regulation endows two isoforms of the transcription factor LIN-29 with distinct functions to achieve coordinated execution of separate juvenile-to-adult transition events in Caenorhabditis elegans..
In-planta ancestral protein resurrection of the female determinant of self-incompatibility specificity in Arabidopsis halleri demonstrates that two allelic variants currently segregating as distinct receptor-ligand combinations diverged through an asymmetrical process.
Endoplasmic reticulum proteostasis factors enhance the mutational tolerance of influenza hemagglutinin, a model secretory pathway protein and therapeutic target, particularly improving the fitness of temperature-sensitive variants.
Investigation of synapse development using a single neuron system illuminates how individual neurons specify connectivity with their postsynaptic partners and the central role of the synaptic organizer neurexin in this process.
The METHYL-CpG-BINDING DOMAIN 7 (MBD7) complex promotes the activation (rather than repression) of transgenes that undergo DNA methylation and it does so without significantly altering their methylation status, placing this complex downstream of DNA methylation.
Analyses of a developmentally regulated Drosophila myofiber remodeling program provide insight into induced autophagy required for T-tubule membrane reorganization, and uncover a conserved Rab2 role in autophagosome-lysosome fusion.