Single-neuron synaptic imaging in vivo demonstrates that highly conserved molecules such as UNC-13, UNC-18, and protein kinase C, which are found at essentially every synapse make different functional contributions in Caenorhabditis elegans glutamatergic neurons.

Genetic and pharmacological tools are used to dissect the mechanisms by which the presynaptic actin cytoskeleton facilitates membrane internalization at synapses and, thereby, neurotransmission.

Single synaptic vesicle imaging shows that kinetically distinct endocytic pathways are differentially regulated by calcium and temperature, and influence the fidelity of synaptic vesicle protein retrieval.

Electrophysiological and live-cell imaging analyses identify a presynaptic function of Ca²⁺ influx via transient receptor potential melastatin 7 (TRPM7) channels.

At mammalian synapses the clathrin adaptor AP-2 plays a crucial role in the endocytic retrieval of a subset of synaptic vesicle proteins from the presynaptic cell surface, while clathrin is dispensable.

A combination of tethered diffusion of release-ready synaptic vesicles and vesicle-vesicle fusion supports neurotransmitter release at the presynaptic active zone of sensory synapses.

The family of ANTH domain proteins serve as adaptors to endocytose ubiquitinated proteins from the cell surface.

A switch-like cell fate divergence revealed by continuous single-cell tracking during entry into quiescence.

During viral assembly, ESCRTIII and VPS4 are recruited after most of the HIV-1 membrane is bent but disappear prior to scission of the virion from the cell.

In mitotically aging yeast cells, the cytosol acidifies, the distances between the organellar membranes decrease dramatically, but crowding on the scale of the average size protein is relatively stable.