A new biochemical method tests whether or not the pre-existing RNA structural correlations couple small molecule binding to gene expression in a paradigmatic riboswitch.
Frank Wolfgang Albert, Joshua S Bloom ... Leonid Kruglyak
A genetic mapping study in more than 1000 yeast individuals reveals the complexity of trans-acting genetic influences on transcriptome variation in unprecedented depth and detail.
Single-molecule FRET experiments reveal that G40P, a DnaB-like helicase, unwinds double-stranded DNA in single base pair steps and its processivity is enhanced by host primase DnaG.
William T Ireland, Suzannah M Beeler ... Rob Phillips
A combination of massively parallel reporter assays and mass spectrometry uncovers the regulation of previously unexplored promoters across the Escherichia coli genome.
Building on previous work (Skene et al., 2014), we show that a new ChIP-seq protocol provides superior resolution and ease of use at low sequence depth of coverage for generating genome-wide maps of protein binding.
Computational analysis of MNase-seq data reveals that alternatively positioned nucleosomes are prevalent in both yeast and human cells and create significant heterogeneity within cell populations.
The rate of DNA unwinding by RecQ helicases is dramatically modulated by the DNA duplex stability in a geometry-dependent manner, providing an intrinsic mechanism for suppressing illegitimate recombination.
Shalini Gupta, Larry J Friedman ... Stephen P Bell
A single origin–recognition complex (ORC) directs loading of a pair of head-to-head Mcm2-7 replicative DNA helicases by forming a protein tether to the first helicase, releasing from its initial DNA-binding site, and rebinding the origin DNA in the opposite orientation.
Distributed cohesin rings flexibly tether the axial element structure of meiotic chromosomes to the underlying chromatin to readily accommodate ongoing transcription.
