Knockout of both latrophilin-2 and latrophilin-3 from Purkinje cells in mice selectively impairs parallel-fiber synapses, revealing a redundant but critical function for latrophilins at specific synapses.
Motor training decreased the unit synaptic response and suppressed induction of long-term depression at parallel fiber to Purkinje cell synapses in the cerebellum, supporting involvement of long-term depression in motor learning.
During parallel fibre activity in vivo, postsynaptic mGluR1 receptors in molecular layer interneurons of the cerebellar cortex are engaged in a frequency-dependent manner and in concert with inotropic glutamate receptors.
The mobilization or silencing of two heterogeneous pools of synaptic vesicles via different frequencies probably enables granule cell to Purkinje cell synapses to better discriminate between the high-rate code of sensory information and background noise.
In vivo recordings in unanesthetized zebrafish larvae show that Purkinje neurons have two stable membrane potential states and that climbing fiber inputs can toggle them to up states during motor episodes.
Physiological and behavioral analyses show that expression of cerebellar whisker learning can be mediated by increased simple spike activity, depending on LTP induction at parallel fiber to Purkinje cell synapses.
In behaving mice, inhibition from molecular layer interneurons attenuates excitation of Purkinje cells by parallel fibers and suppresses their ability to enhance climbing fiber-triggered dendritic Ca2+ responses.