2D template matching complements 3D template matching of tomograms, providing fast and specific detection of molecules in cryo-EM images of cells, as well as generating 3D reconstructions from detected targets.
Adenoviral CRISPR/Cas9 gene knockdown of mutant Col8a2 achieves structural and functional rescue of murine Fuchs’ endothelial corneal dystrophy, the first demonstration of post-mitotic CRISPR-based knockdown for phenotypic restoration.
Precise motor control is supported by connectivity within a cortical grasping network from childhood to adulthood, but older individuals display more top-down control and superior motor performance compared to children.
Combined genetic experiments and cellular trafficking assays in Drosophila and mammalian models uncover a new role of RAS-like GTPases in EGFR signalling activation during tissue regeneration and tumourigenesis through regulation of EGFR internalisation.
The transcriptional landscape of epicardial cells revealed 11 cell populations that differentially expressed early cardiac genes and numerous cardioprotective paracrine factors defining their regenerative potential.