Browse the search results

Directed evolution of the resistance enzyme Cfr under antibiotic selection identifies increased Cfr expression and stability as strategies to boost resistance and reveals that Cfr modification of the ribosome confers resistance by sterically occluding binding of antibiotics.

GTPBP7, NSUN4, and MTERF4 facilitate maturation of the peptidyl transferase center of the mitochondrial ribosome.

Cryo-EM and functional studies reveal how combined action of proteins RPS26/eS26 and RIO1 allows late precursors to the human small ribosomal particle to be matured into fully translation-competent 40S subunits.

The cotranslational membrane integration of three multispanning Escherichia coli inner membrane proteins is followed using force profile analysis, uncovering unexpected complexities in the membrane integration process.

Natural substrates of the central endoplasmic reticulum quality control glycoprotein sensors UDP-glucose:glycoproteinglucosyltransferase (UGGT)1 and UGGT2 were identified using a glycoproteomics approach and the role for their modification was explored.

In nematode worms, NSUN-1 methylates ribosomal RNA and influences phenotypes related to aging, stress resistance, germ line development, and cuticle integrity by regulating translation of specific mRNAs.

HemK NTD cotranslational folding starts within the ribosome exit tunnel upon N-terminal helix synthesis and proceeds sequentially through a series of intermediates becoming less dynamic as the nascent chain grows.

High-resolution maps and models of the bacterial ribosome provide new chemical insights into protein synthesis, and should enable the development of robust tools for cryo-EM structure modeling and refinement.

Inhibition of bacterial protein synthesis by an antimicrobial peptide apidaecin triggers translation arrest at the stop codons, ribosome queuing and pervasive stop codon readthrough.

Methods that use puromycin to localize active translation do not actually detect the site of translation.