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    1. Computational and Systems Biology

    Repurposing the mammalian RNA-binding protein Musashi-1 as an allosteric translation repressor in bacteria

    Roswitha Dolcemascolo, María Heras-Hernández ... Guillermo Rodrigo
    A mammalian protein with RNA recognition motifs can be used in bacteria to engineer post-transcriptional regulation.
    1. Evolutionary Biology
    2. Genetics and Genomics

    Comprehensive fitness maps of Hsp90 show widespread environmental dependence

    Julia M Flynn, Ammeret Rossouw ... Daniel NA Bolon
    Environmental conditions strongly impact the fitness effects of Hsp90, resulting in the selection of Hsp90 sequences in nature that are robust to a variety of stressful conditions.
    1. Computational and Systems Biology

    Statistical analysis supports pervasive RNA subcellular localization and alternative 3' UTR regulation

    Rob Bierman, Jui M Dave ... Julia Salzman
    Analysis of genome-wide spatial transcriptomics data reveals cell-type specific subcellular RNA localization, and a subset of genes show significantly high correlation between spatial patterning and 3' UTR length.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Genome rearrangements and pervasive meiotic drive cause hybrid infertility in fission yeast

    Sarah E Zanders, Michael T Eickbush ... Harmit Singh Malik
    Rapid acquisition of chromosome rearrangements, together with independently acting transmission distorter alleles on each chromosome, drive near complete sterility in fission yeast hybrids.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    Bridged filaments of histone-like nucleoid structuring protein pause RNA polymerase and aid termination in bacteria

    Matthew V Kotlajich, Daniel R Hron ... Robert Landick
    Bridged H-NS filaments inhibit transcript elongation by bacterial RNA polymerase by enhancing backtracking and increasing Rho-dependent termination at a subset of pause sites that are normally poor Rho substrates.
    1. Ecology

    Genomic adaptations in information processing underpin trophic strategy in a whole-ecosystem nutrient enrichment experiment

    Jordan G Okie, Amisha T Poret-Peterson ... James J Elser
    Trait-based metagenomic analysis of an ecosystem fertilization experiment shows that genomic traits that affect the costs and rates of biochemical information-processing mediate community assembly.
    1. Cell Biology

    Tom70-based transcriptional regulation of mitochondrial biogenesis and aging

    Qingqing Liu, Catherine E Chang ... Chuankai Zhou
    Tom70 connects the mitochondrial import to the nuclear transcriptional activity of mitochondrial proteins and controls mitochondrial biogenesis defects during aging.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Live-cell mapping of organelle-associated RNAs via proximity biotinylation combined with protein-RNA crosslinking

    Pornchai Kaewsapsak, David Michael Shechner ... Alice Y Ting
    Live-cell nanometer-resolution RNA labeling method enables transcriptome-wide mapping of endogenous RNAs in nuclear, cytosol, ER, and mitochondrial subcompartments.
    1. Computational and Systems Biology

    Phenotypic diversity and temporal variability in a bacterial signaling network revealed by single-cell FRET

    Johannes M Keegstra, Keita Kamino ... Thomas S Shimizu
    Noise in a signaling network comprising thousands of molecules shapes diversity across cell populations and generates giant temporal fluctuations at the single-cell level.
    1. Cell Biology
    2. Chromosomes and Gene Expression

    Many lncRNAs, 5’UTRs, and pseudogenes are translated and some are likely to express functional proteins

    Zhe Ji, Ruisheng Song ... Kevin Struhl
    Thousands of "noncoding" RNAs, 5' "untranslated" regions, and pseudogenes in humans are actually translated, and some of these are likely to express functional proteins.