Synthetic single domain antibody libraries and a binder selection cascade encompassing ribosome and phage display enable the selection of conformation-specific binders against previously intractable membrane proteins within three weeks.
Humans with mutations in the AIRE gene exhibit common autoantibodies targeting ovarian and intestinal antigens, including intestinal dysfunction-associated antibodies to enteroendocrine transcription factor RFX6.
Nanobodies are developed that target neuronal proteins with restricted subcellular distributions and used as intrabodies to deliver cargo to specific sites within neurons and nanoscale immunolabels for improved imaging resolution.
Direct cortical recordings in humans link the spectral structure of local field potentials to inhibition/disinhibition mechanisms coordinating sensorimotor neuronal populations during movement imagery.
Escherichia coli is surprisingly tolerant to chromatinization by archaeal histones, suggesting that histones can become established as ubiquitous chromatin proteins without interfering critically with some key DNA-templated processes.