The Volta phase plate enables in-focus single particle analysis at near atomic resolutions, which could expand the capabilities of cryo-EM, especially for small, flexible or heterogeneous macromolecular samples.
Radostin Danev, Dimitry Tegunov, Wolfgang Baumeister
Building on previous work in cryo-electron microscopy (Entchev et al, 2015), it shown that a combination of the Volta phase plate and a small amount of defocus can simplify the experimental set-up, increase the data acquisition rate and improve resolution.
Cryo electron tomography provides the first high-resolution 3D axoneme structure from any pathogenic organism, revealing novel structures that support the unique motility of these pathogens through host tissues.
Adaptation within a continuous attractor neural network explains theta phase precession and procession, providing insight into the neural mechanism of theta phase coding in the hippocampus.
Nina Kirstein, Alexander Buschle ... Aloys Schepers
Replication origins are established throughout the genome with the exception of transcribed genes, and the local chromatin composition likely modulates the density of ORC and MCM as well as origin activation.
Human hippocampal connectivity to network afferents varies continuously with the phase of the local theta oscillation, confirming a putative mechanism by which neural oscillations modulate human hippocampal function.
A computational framework and analysis of rodent CA1 data reveals that theta oscillations sequentially sweep through the past, present, and future at a spatial scale that varies with the characteristic speed of the animal.
Angus Chadwick, Mark CW van Rossum, Matthew F Nolan
A new model for phase precession accounts for theta sequence generation, suggests critical roles for interneurons, and predicts circuit properties to optimise memory storage.
Matthias Pöge, Julia Mahamid ... Wolfgang Baumeister
Cryo-electron tomography of close-to-native rod outer segments resolves connectors between disks and a scaffold at the disk rim enforcing the high membrane curvature of the unique long-range organization in these rhodopsin-rich light-sensitive subcellular organelles.
