39 results found
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Dynamics of the IFT machinery at the ciliary tip

    Alexander Chien, Sheng Min Shih ... Ahmet Yildiz
    During intraflagellar transport (IFT), kinesin-II dissociates from IFT trains at the flagellar tip and its diffusion in flagella serves as a negative feedback mechanism that facilitates flagellar length control in Chlamydomonas.
    1. Cell Biology

    Super-resolution microscopy reveals majorly mono- and dimeric presenilin1/γ-secretase at the cell surface

    Abril Angélica Escamilla-Ayala, Ragna Sannerud ... Wim Annaert
    Super-resolution microscopy sets a new strategy to comprehend the membrane organization of γ-secretase at single complex resolution identifying nanodomain associations and its diffusion in situ in the living membrane.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Intraflagellar transport: Trainspotting in a cilium

    Dhivya Kumar, Stephen M King
    A new imaging technique sheds light on how cilia regulate their length and growth.
    Version of Record
    Insight
    1. Biochemistry and Chemical Biology

    Optical tools for visualizing and controlling human GLP-1 receptor activation with high spatiotemporal resolution

    Loïc Duffet, Elyse T Williams ... Tommaso Patriarchi
    A genetically encoded sensor and a photocaged peptide together enable precise investigation of human glucagon-like peptide-1 receptor activation with high spatiotemporal resolution.
    1. Genetics and Genomics

    Firefly genomes illuminate parallel origins of bioluminescence in beetles

    Timothy R Fallon, Sarah E Lower ... Jing-Ke Weng
    The first genomic view of beetle luciferase evolution indicates evolutionary independence of luciferase between fireflies and click-beetles, and provide valuable datasets which will accelerate the discovery of new biotechnological tools.
    1. Neuroscience

    Optical control of pain in vivo with a photoactive mGlu5 receptor negative allosteric modulator

    Joan Font, Marc López-Cano ... Francisco Ciruela
    Localized drug photoactivation provides light-mediated analgesia in behaving mice.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Structural basis of proton translocation and force generation in mitochondrial ATP synthase

    Niklas Klusch, Bonnie J Murphy ... Werner Kühlbrandt
    Cryo-EM reveals atomic details of two membrane half-channels for translocation of protons that drive rotary catalysis in ATP synthase.
    1. Structural Biology and Molecular Biophysics

    Revealing an outward-facing open conformational state in a CLC Cl/H+ exchange transporter

    Chandra M Khantwal, Sherwin J Abraham ... Merritt Maduke
    The characterization of a previously unidentified “outward-facing open” conformational state provides a new framework for understanding the CLC transport mechanism.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Structural characterization of ligand binding and pH-specific enzymatic activity of mouse Acidic Mammalian Chitinase

    Roberto Efraín Díaz, Andrew K Ecker ... James S Fraser
    Biochemical and structural investigations of the pH adaptability of mouse Acidic Mammalian Chitinase offer insights for designing enhanced enzyme variants that can function in both lung and gut.
    1. Neuroscience

    LabNet hardware control software for the Raspberry Pi

    Alexej Schatz, York Winter
    A software distributed platform for the Raspberry Pi to simplify and unify hardware access over the Ethernet network with almost real-time latencies.

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