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26 results found
    1. Structural Biology and Molecular Biophysics

    Monochromatic multicomponent fluorescence sedimentation velocity for the study of high-affinity protein interactions

    Huaying Zhao et al.
    Fluorescence detected sedimentation velocity offers a new method for studying heterogeneous protein interactions in solution by exploiting characteristic temporal signal modulations of photoswitchable fluorescent proteins.
    1. Biochemistry and Chemical Biology
    2. Neuroscience

    Erasable labeling of neuronal activity using a reversible calcium marker

    Fern Sha et al.
    Protein engineering of a reversibly switchable fluorescent protein enables post-hoc reversible and repeatable marking of elevated calcium concentrations using blue light.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Importin-β modulates the permeability of the nuclear pore complex in a Ran-dependent manner

    Alan R Lowe et al.
    The Ran GTPase plays a role in defining the physical properties of the nuclear pore complex transport channel by remodeling the binding interactions of importin-β with the nucleoporin Nup153 at the nuclear face of the pore.
    1. Biochemistry and Chemical Biology

    Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides

    Matthijs Kol et al.
    Photoswitchable ceramides containing an azobenzene photoswitch behave as light-controllable substrates for both sphingomyelin synthase and glucosylceramide synthase.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Dynamics of the IFT machinery at the ciliary tip

    Alexander Chien et al.
    During intraflagellar transport (IFT), kinesin-II dissociates from IFT trains at the flagellar tip and its diffusion in flagella serves as a negative feedback mechanism that facilitates flagellar length control in Chlamydomonas.
    1. Cell Biology
    2. Plant Biology

    Delivery of endocytosed proteins to the cell–division plane requires change of pathway from recycling to secretion

    Sandra Richter et al.
    The same membrane trafficking cargo can change pathways mediated by different members of ARF-GEF family of vesicle formation regulators.
    1. Cell Biology
    2. Neuroscience

    Small-molecule G-quadruplex stabilizers reveal a novel pathway of autophagy regulation in neurons

    Jose F Moruno-Manchon et al.
    G-quadruplex DNA regulates autophagy in neurons.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Optogenetic activation of heterotrimeric G-proteins by LOV2GIVe, a rationally engineered modular protein

    Mikel Garcia-Marcos et al.
    LOV2GIVe allows to activate Gi proteins non-invasively with innocuous blue light based on a design principle unrelated to light-activated GPCRs (metazoan opsins), thereby expanding the range of potential experimental applications.
    1. Structural Biology and Molecular Biophysics

    rsEGFP2 enables fast RESOLFT nanoscopy of living cells

    Tim Grotjohann et al.
    The discovery of a fluorescent protein that can be rapidly switched between long-lived ‘on’ and ‘off’ states will lead to a new generation of super-resolution imaging experiments on living cells.

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