A panel of chimpanzee induced pluripotent stem cells (iPSCs) will help realise the potential of iPSCs in primate studies, and in combination with genomic technologies, transform studies of comparative evolution.

A post-translational modification called O-GlcNAcylation modulates the activity of the SOX2 transcription factor in pluripotent stem cells.

OCT4 and SOX2 display partially independent activity to regulate chromatin accessibility, and highly dynamic activity of OCT4 is required throughout the cell cycle to maintain pluripotency enhancer accessibility.

Identifying the pathways that support human naive-state pluripotent stem cells provides insights into the signalling-based regulation of human pluripotency and enables informed decisions to improve conditions for pluripotent cell culture.

Genetic manipulations show that endogenous transcription factors of the SoxB1 class act redundantly to maintain primed pluripotency and reveal differential effects on transitions between pluripotent and differentiation states.

Naive hPSCs can readily give rise to human trophoblast stem cells, thus demonstrating their extraembryonic lineage potential and providing a new model system to study human trophectoderm specification.

Clonal naïve human embryonic stem cells assemble an Arp2/3 complex-dependent contractile actin filament ring around colonies that promotes tensional forces favoring cell-cell adhesion and nuclear translocation of YAP to maintain ground-state pluripotency.

RNA-binding protein SRSF3 mediates critical changes in RNA processing of pluripotency genes, which reveals functional consequences of regulated RNA processing during stem cell self-renewal and early development.

Adding suppressors of spontaneous differentiation enables precise control of human induced pluripotent stem cell (hiPSC) status in suspension culture conditions and leads to scalable and automated cell therapy using hiPSCs.

Micropatterned differentiation of mouse pluripotent stem cells gives rise to regionally distinct cell types arising in embryos at gastrulation.