A panel of chimpanzee induced pluripotent stem cells (iPSCs) will help realise the potential of iPSCs in primate studies, and in combination with genomic technologies, transform studies of comparative evolution.
Genetic manipulations show that endogenous transcription factors of the SoxB1 class act redundantly to maintain primed pluripotency and reveal differential effects on transitions between pluripotent and differentiation states.
OCT4 and SOX2 display partially independent activity to regulate chromatin accessibility, and highly dynamic activity of OCT4 is required throughout the cell cycle to maintain pluripotency enhancer accessibility.
RNA-binding protein SRSF3 mediates critical changes in RNA processing of pluripotency genes, which reveals functional consequences of regulated RNA processing during stem cell self-renewal and early development.
Identifying the pathways that support human naive-state pluripotent stem cells provides insights into the signalling-based regulation of human pluripotency and enables informed decisions to improve conditions for pluripotent cell culture.
Naive hPSCs can readily give rise to human trophoblast stem cells, thus demonstrating their extraembryonic lineage potential and providing a new model system to study human trophectoderm specification.