The super-resolution fluorescence microscopy approach polarization PALM (p-PALM) reveals that macromolecular crowding and inhomogeneity within nuclear pores generate a structurally and dynamically complex permeability barrier.

The present fluorescence polarization microscopy method enables the determination of the spatial orientations and lifetimes of a transporter protein in individual conformational states required for understanding the complex conformational mechanism underlying its function, applicable to investigation of other membrane proteins.

Asymmetric tension increase along the cell equator promotes self-organization of actomyosin into a partially aligned network during cytokinetic cleavage furrow ingression of vertebrate cells.

Advances in quantitative phase and polarized light microscopy, combined with deep learning, reveal the architecture of human brain tissue.

Kinesin-4 KIF21B promotes rapid reorientation of the microtubule network during formation of immunological synapse in T cells by acting as a pausing and catastrophe-inducing factor that keeps microtubules short.

Cryogenic super-resolution microscopy resolves the three-dimensional arrangement of individual fluorescent markers attached to protein complexes with Angstrom precision through their blinking behavior, polarization selection and a classification scheme.

The discovery of evolutionarily conserved macrophage subsets in zebrafish paves the way for a comprehensive study of macrophage behaviour and function.

Proteins pivotal to epithelial polarization organize in separated clusters, simplifying the current view by showing that a small fraction of the many protein–protein interactions proposed are prominent in mature epithelia.

Compositional changes alter actin binding by phase separated T cell signaling clusters, enabling cluster movement by distinct actin networks.

Centrosomal Aurora A (AIR-1), together with cortical actomyosin flows, induces polarization of ECT-2, the activator of RHO-1, during polarization and cytokinesis, in order to promote furrow formation.