Yeast promoters can harbor multiple natural DNA variants that influence gene expression, interact genetically, evolve under negative selection, alter transcription factor motifs, and remain challenging to predict.
Transcriptome-scale RNA imaging and lifetime measurements reveal that the E. coli transcriptome is spatially organized and that this organization modulates the post-transcriptional fate of bacterial mRNAs.
Crosslink immunopreciptiation (iCLIP) studies reveal important mechanistic insights into how MARF1 post-transcriptionally regulates targeted mRNAs and uncover a novel mode by which EDC4 regulates mRNA metabolism.
Time resolved crystal structures reveal the binding of activated monomers to the template, followed by generation of the imidazolium-bridged dinucleotide intermediate, and finally formation of a phosphodiester bond between the primer and the adjacent monomer.
The diffusion coefficients of proteins in the cytoplasm depend on their net charge and the distribution of charge over the protein surface, with positive proteins moving up to 100-fold slower because they bind to ribosomes.
The general transcription elongation factor NusG functions as an intrinsic termination factor in Bacillus subtilis and together with NusA coordinates global gene expression including the motility regulon.