Short homology arms exposed by CRISPR/Cas9 cleavage can target integration at genomic CRISPR/Cas9 cut sites at high frequencies with reproducible precision using pGTag vectors in zebrafish and mammalian cells.
Drosophila synaptotagmin 7 functions to restrict SV availability and release, but does not act as the Ca2+ sensor mediating the asynchronous release and facilitation remaining in synaptotagmin 1 mutants.
Networks simulations and in vivo imaging suggest a stable backbone of stimulus representation formed by neurons with low population coupling, alongside a flexible substrate of neurons with high population coupling.
A new analysis algorithm (DISC) enables accurate analysis of data from high-throughput single-molecule paradigms and reveals a non-cooperative binding mechanism of cyclic nucleotide-binding domains from HCN ion channels.
Collective responses of animals are generally controlled by complex biological mechanisms and in Caenorhabditis eleganscollective dynamics are purely controlled by physical parameters such as oxygen penetration and bacterial diffusion.