Calculations of flagellar energetics from beating patterns in sperm reveal that internal dissipation by dynein motors and other passive structures within the flagellum significantly exceeds external hydrodynamic dissipation.
The cryo-EM structures of actomyosin-V, including a novel strongly bound post-rigor transition state, reveal a pronounced structural heterogeneity of myosin-V and the specific selection of the closed D-loop conformation of F-actin.
Romain F Laine, Gemma Goodfellow ... Clemens F Kaminski
Machine learning in conjunction with super-resolution imaging allows for the first time to quantitatively analyse large and heterogenous virus samples structure at a high throughput and specificity.
A high-throughput analysis of several self-cleaving ribozymes reveals the effect of every possible mutation, and every possible pair of mutations, and how patterns in the relative activity data can be mapped to canonical and non-canonical structural elements.
Krishna Chinthalapudi, Sarah M Heissler ... Dietmar J Manstein
R788 is part of an allosteric communication pathway that connects the converter at the distal end of the myosin motor domain via the relay helix with switch-2 of the active site.
Empirical energy landscape allows simulating the structural dynamics of the proteasomal ATPase complex, which yields predictions that are widely consistent with experimental observations and reveals the functional mechanism of the proteasome in substrate degradation.
Ricardo Guerrero-Ferreira, Nicholas MI Taylor ... Henning Stahlberg
The alpha-synuclein fibril structure reported here buries residues 50-57 at the interface between its two protofilaments, suggesting that familial Parkinson's disease associated mutations in these residues lead to a structure not compatible with the one presented here.
