Localized temperature gradients used as a novel method of active micro-theology that is non-invasive and probe-free, to investigate material responses of cell departments such as the different compartments of a nucleus displaying distinct material properties.

A reusable system of 3D printed stainless steel microdrive and plastic head cap allows stable recording of electrophysiological data in mice and rats and easy recovery of silicon probes.

A biophysically principled algorithm can build quantitative models of protein-DNA binding specificity of unprecedented accuracy from a leading type of high-throughput in vitro binding data.

A new combined mechanism explains how GTP binding by tubulin is linked to its dynamics and energetics in solution and why GTP-induced flexibility, and not the tubulin conformation as such, drives microtubule assembly.

Cytosolic and organellar Hsp90s from higher eukaryotes have evolved a variable, and environmentally responsive N-terminal extension to regulate their activity.

Single molecule FRET reveals that the SWI/SNF-family remodeling enzyme RSC translocates nucleosomes along the DNA in steps of ~1-2 base pairs in size.

A new FRET-based cAMP biosensor with nanomolar sensitivity makes it possible to measure cAMP dynamics in miniscule subcellular compartments like cilia and flagella.

Time-resolved transition metal ion fluorescence resonance energy transfer using a fluorescent, noncanonical amino acid donor and metal ion acceptor measures distances and distance distributions in proteins, allowing the equilibrium distributions among conformational states to be accurately determined.

Preconditioned cues provide information about an associative model but do not, by default, trigger representations of value, either model-based or model-free.

A family of fluorescent biosensors for nicotinamide adenine dinucleotides allows quantification of these cofactors in live cells with spatio-temporal resolution.