Divergent Cas9 enzymes direct site-specific single-stranded RNA cleavage, reducing infection by RNA phage in vivo and enabling programmable, PAM-independent repression of gene expression in bacteria.
Lentiviral protein transduction offers a new approach for administration of custom-designed nucleases, leading to high-efficacy targeted gene editing and reduced off-target activity after virus-directed delivery of programmable nuclease proteins.
An EASAC working group on genome editing recommends that regulators should focus on specific applications of these new techniques rather than attempting to regulate genome editing itself as a new technology.
We discuss the methods available to understand lncRNA function in vivo, and highlight important considerations that should be taken into account when designing such experiments.
Adiponectin signaling integrates metabolic state information to regulate circadian clock function in hypothalamic appetite regulating centers, food intake rhythms, and body weight.
Sex-specific characteristics of the fruit fly courtship behavior are not specified by a single binary switch, but as a combination of traits that are modularly specified by separable genetic switches.
Male-type aggressive and courtship behaviors of the fruit flies are differentially specified by two sex-determining genes, providing a substrate for the evolution to sculpt these two behaviors independently.
