Divergent Cas9 enzymes direct site-specific single-stranded RNA cleavage, reducing infection by RNA phage in vivo and enabling programmable, PAM-independent repression of gene expression in bacteria.
Lentiviral protein transduction offers a new approach for administration of custom-designed nucleases, leading to high-efficacy targeted gene editing and reduced off-target activity after virus-directed delivery of programmable nuclease proteins.
An EASAC working group on genome editing recommends that regulators should focus on specific applications of these new techniques rather than attempting to regulate genome editing itself as a new technology.
We discuss the methods available to understand lncRNA function in vivo, and highlight important considerations that should be taken into account when designing such experiments.
A method was developed to non-invasively and simultaneously track individual action-potentials propagating across multiple branches of identified neurons in neocortical cultures.
The helical rod structure and dynamic spring-like properties of the type 1 pilus are evolutionarily fine-tuned for functioning in host-pathogen interactions during urinary tract infection and gut colonization.
Nucleosomes provide a direct and profound block to the activity of the CRISPR effector protein Cas9, suggesting future sophisticated design rules for CRISPR targeting.
Cocktail-party listening performance in normal-hearing listeners is associated with the ability to focus attention on a target stimulus in the presence of distractors.
Temperature-activated TRPV1 ion channels respond to increased temperatures by opening and then entering an inactivated state from which they cannot recover, suggesting that this form of irreversible gating results from partial unfolding during heat absorption.
