Divergent Cas9 enzymes direct site-specific single-stranded RNA cleavage, reducing infection by RNA phage in vivo and enabling programmable, PAM-independent repression of gene expression in bacteria.

Lentiviral protein transduction offers a new approach for administration of custom-designed nucleases, leading to high-efficacy targeted gene editing and reduced off-target activity after virus-directed delivery of programmable nuclease proteins.

Horizon scanning has been used to identify opportunities and risks presented by 20 emerging issues in the field of biological engineering.

An EASAC working group on genome editing recommends that regulators should focus on specific applications of these new techniques rather than attempting to regulate genome editing itself as a new technology.

We discuss the methods available to understand lncRNA function in vivo, and highlight important considerations that should be taken into account when designing such experiments.

The helical rod structure and dynamic spring-like properties of the type 1 pilus are evolutionarily fine-tuned for functioning in host-pathogen interactions during urinary tract infection and gut colonization.

The PIE-scope resolves the limitations in correlative cryo-focused ion beam milling by adding robustness and ease of use to the workflow.

Motif length plays a critical role in the specification of biological function within a conserved family of RNA-binding proteins.

Linking the DNA repair template to the Cas9 ribonucleoprotein complex enhances homology-directed repair of the induced DNA double strand break.

Gene knock-out of the omega-1 ribonuclease of Schistosoma mansoni eggs resulted in immunologically impaired phenotype, showcasing the novel application of CRISPR/Cas9 genome editing and utility for functional genomics in schistosomes.