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    1. Genetics and Genomics

    Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors

    Joseph M Replogle, Jessica L Bonnar ... Marco Jost
    Newly developed, highly active CRISPR interference single guide RNA libraries and effectors together enable high-quality genetic screens across cell models.
    1. Biochemistry and Chemical Biology
    2. Ecology

    Green fluorescent protein-like pigments optimise the internal light environment in symbiotic reef-building corals

    Elena Bollati, Niclas H Lyndby ... Daniel Wangpraseurt
    Scalar irradiance microsensor measurements performed inside the tissue of living corals show that absorption and fluorescence emission by host pigments produce dramatic spectral alterations in the light environment experienced by the symbionts.
    1. Computational and Systems Biology
    2. Neuroscience

    A hardware system for real-time decoding of in vivo calcium imaging data

    Zhe Chen, Garrett J Blair ... Hugh T Blair
    DeCalciOn is a low-cost open-source hardware system for real-time in vivo calcium imaging that offers capabilities for online decoding of neural population activity and delivery of short latency closed-loop feedback in freely behaving animals.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Antibodies to repeat-containing antigens in Plasmodium falciparum are exposure-dependent and short-lived in children in natural malaria infections

    Madhura Raghavan, Katrina L Kalantar ... Joseph L DeRisi
    The dominant targets of host immune response to malaria are repeat elements, which are short stretches of amino acids repeated within Plasmodium falciparum proteins, and these are associated with short-lived and exposure-dependent antibody responses.
    1. Genetics and Genomics

    Characterization of full-length CNBP expanded alleles in myotonic dystrophy type 2 patients by Cas9-mediated enrichment and nanopore sequencing

    Massimiliano Alfano, Luca De Antoni ... Marzia Rossato
    A novel sequencing-based method to characterize CNBP microsatellite expansions in DM2 patients demonstrates benefits for an improved dissection of DM2 genetic architecture, thus potentially ameliorating patient stratification and genetic counseling.
    1. Cancer Biology
    2. Medicine

    Open-top Bessel beam two-photon light sheet microscopy for three-dimensional pathology

    Won Yeong Park, Jieun Yun ... Ki Hean Kim
    Open-top Bessel beam two-photon light sheet microscopy have the potential for rapid and accurate nondestructive pathology by visualizing 3D histological information.
    1. Ecology

    Point of View: A transatlantic perspective on 20 emerging issues in biological engineering

    Bonnie C Wintle, Christian R Boehm ... William J Sutherland
    Horizon scanning has been used to identify opportunities and risks presented by 20 emerging issues in the field of biological engineering.
    1. Computational and Systems Biology
    2. Neuroscience

    High-throughput automated methods for classical and operant conditioning of Drosophila larvae

    Elise C Croteau-Chonka, Michael S Clayton ... Kristina T Klein
    A novel high-throughput FPGA-based multi-animal tracking and training system was used to demonstrate trace and operant conditioning in Drosophila larvae.
    1. Cancer Biology
    2. Cell Biology

    A versatile system to record cell-cell interactions

    Rui Tang, Christopher W Murray ... Monte M Winslow
    A novel reporter system that employs cell surface antigen-nanobody pairing enables physical interaction-dependent labeling and functional modification between diverse cell types.
    1. Neuroscience

    Stretching the skin immediately enhances perceived stiffness and gradually enhances the predictive control of grip force

    Mor Farajian, Raz Leib ... Ilana Nisky
    Elucidating the contribution of augmented artificial skin-stretch stimulation to the fingertips to the immediate illusion of a higher stiffness and to an increased predictive grip force control.