Evolving E. coli NfsA for enhanced chloramphenicol detoxification in the native host environment shows that eliminating substrate competition can occur rapidly, and be key to evolving a new enzymatic function.
A new software developed for high-throughput antibody, T cell receptor, and MHC repertoire analysis uncovers neutrality of the binding interface and intramolecular crosstalk as distinguishing properties of polyreactive antibodies.
Biochemical analysis and computational modeling reveal how cells mechanistically control the quality of their proteomes and demonstrate that the precise alignment of subunits in oligomeric complexes can profoundly affect enzymatic properties.
A gain-of-function in a new chemical defense resulted in no trade-offs and and independent evolution between novel and ancestral defenses, suggesting low redundancy among different defensive chemicals.
A model for synchronous neurotransmitter release suggests that when not in the presence of calcium ions, Synaptotagmin proteins form ring-like structures between the vesicle and plasma membrane that prevent spontaneous fusion.