209 results found
    1. Cell Biology
    2. Physics of Living Systems

    Golgi compartments enable controlled biomolecular assembly using promiscuous enzymes

    Anjali Jaiman, Mukund Thattai
    By partitioning promiscuous enzymes across multiple Golgi compartments, eukaryotic cells can tightly control the synthesis of complex sugar oligomers.
    1. Biochemistry and Chemical Biology

    Underground isoleucine biosynthesis pathways in E. coli

    Charles AR Cotton et al.
    Upon deletion of threonine deaminases, biosynthesis of isoleucine is rescued by two promiscuous reactions, one emerges under aerobic conditions and the other is naturally active under anaerobic conditions.
    1. Evolutionary Biology
    2. Genetics and Genomics

    Mutations that improve efficiency of a weak-link enzyme are rare compared to adaptive mutations elsewhere in the genome

    Andrew B Morgenthaler et al.
    Mutations elsewhere in the genome play critical roles in improving fitness during amplification and divergence of a gene encoding a weak-link enzyme whose inefficiency limits growth rate.
    1. Structural Biology and Molecular Biophysics
    2. Evolutionary Biology

    An atomic-resolution view of neofunctionalization in the evolution of apicomplexan lactate dehydrogenases

    Jeffrey I Boucher et al.
    The convergent evolution of unusually strict substrate specificity in apicomplexan LDHs arose by classic neofunctionalization of a duplicated MDH gene via few mutations of large effect.
    1. Biochemistry and Chemical Biology
    2. Evolutionary Biology

    Intracellular complexities of acquiring a new enzymatic function revealed by mass-randomisation of active-site residues

    Kelsi R Hall et al.
    Evolving E. coli NfsA for enhanced chloramphenicol detoxification in the native host environment shows that eliminating substrate competition can occur rapidly, and be key to evolving a new enzymatic function.
    1. Genetics and Genomics

    Firefly genomes illuminate parallel origins of bioluminescence in beetles

    Timothy R Fallon et al.
    The first genomic view of beetle luciferase evolution indicates evolutionary independence of luciferase between fireflies and click-beetles, and provide valuable datasets which will accelerate the discovery of new biotechnological tools.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    Polymerase θ is a robust terminal transferase that oscillates between three different mechanisms during end-joining

    Tatiana Kent et al.
    Polymerase θ is among the most proficient terminal transferases known and switches between three different mechanisms of terminal transferase activity.
    1. Ecology
    2. Microbiology and Infectious Disease

    A novel ATP dependent dimethylsulfoniopropionate lyase in bacteria that releases dimethyl sulfide and acryloyl-CoA

    Chun-Yang Li et al.
    A novel dimethylsulfoniopropionate lyase was identified, which catalyzes dimethyl sulfide releasing by a new mechanism and is found in several bacterial lineages, revealing its important roles in global sulfur cycling.
    1. Biochemistry and Chemical Biology

    Protomer alignment modulates specificity of RNA substrate recognition by Ire1

    Weihan Li et al.
    Biochemical analysis and computational modeling reveal how cells mechanistically control the quality of their proteomes and demonstrate that the precise alignment of subunits in oligomeric complexes can profoundly affect enzymatic properties.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Crystal structures of virus-like photosystem I complexes from the mesophilic cyanobacterium Synechocystis PCC 6803

    Yuval Mazor et al.
    The structure of the photosystem I (PSI) complex from Synechocystis is determined, and reaction center subunits engineered to resemble a viral PSI are found to promote promiscuous electron acceptor properties.

Refine your results by:

Type
Research categories