The elongation rate of RNA Polymerase II varies greatly between and along genes, as this enzyme accelerates from stable pausing to rapid elongation within genes, and is influenced by CG-content, exons and chromatin.

The structural architecture of the 4-subunit, RNA-binding human negative elongation factor NELF is derived by a combination of X-ray crystallography and protein crosslinking.

The phosphorylation of tyrosine in the heptad repeat of the C-terminal domain of the largest subunit of RNA polymerase II promotes Ser2 phosphorylation by P-TEFb for pausing release.

TRIM28 was found to be a versatile dual-function latency contributor by bridging both suppressive epigenetic modifications and RNAP II transcriptional-pausing, and can be a novel target to develop latency-reversing agents.

We discuss the methods available to understand lncRNA function in vivo, and highlight important considerations that should be taken into account when designing such experiments.

In vitro and in cellulo characterization of oligomerization by the cytoplasmic Wnt effector dishevelled and its partner Axin provide new mechanistic principles for Wnt/β-catenin signaling.

GTP binding induces rearrangement of S-OPA1 assembly on membrane and thus triggers membrane remodeling with reduced curvature.

Novel insights into LIS1-dependent regulation of cell membrane contractility and cleavage axis specification identify a key molecular network regulating mitoses of neural progenitors and somatic cells during development.

The RAB-28 GTPase regulates ciliary extracellular vesicle shedding, which may be important for neuron-glia communication.

The co-chaperone CHORDC1 is specifically required for epidermal growth factor receptor trafficking and signaling in Caenorhabditis elegans and in human cells.