Structure-led protein engineering can expand the effector recognition profile of plant intracellular NLR immune receptors, providing a proof-of-principle for the development of novel disease resistance mechanisms in plants.
Tn5 transposase has direct tagmentation activity towards RNA/DNA hybrids, which is harnessed as a more convenient and faster RNA-seq library construction method and will benefit RNA and chromatin research.
Computational and theoretical models reveal mechanisms by which protein compartments assemble around enzymes and reagents to facilitate reactions in bacteria, allowing the identification of strategies for reengineering such compartments as customizable nanoreactors.
Fly protein families Dprs and DIPs can create a multitude of complementary interfaces for homo- and heterophilic adhesion complexes, resulting in instructive roles for connectivity in the motor neuron circuitry.
eMags is an engineered photodimerizer pair for optogenetic modulation in mammalian cells that is especially suited for the manipulation of intracellular processes occurring in small volumes or subcellular organelles.
Two structurally-unrelated regulatory proteins utilize parallel molecular mechanisms to selectively tune calcium and calmodulin feedback of calcium and sodium ion channels and reveals a novel strategy to engineer synthetic channel modulators.