Using a suite of CRISPR technologies, unique chemical tools, and carefully designed biochemical and cell biological assays, we define the mechanism of action of Retro-2, an inhibitor of retrograde toxins.
Other than its function in mechanotransduction, TMC1 is indispensable for action potential firing of auditory hair cells by mediating a leak conductance that alters tonotopically along the cochlea coil.
Diurnal variation loss of pineal allopregnanolone synthesis by light-at-night induces pituitary adenylate cyclase-activating polypeptide (PACAP) reduction and transcriptional PACAP repression in the cerebellum, with subsequent Purkinje cell death.
A large-scale, sibling-based analysis of the Icelandic population spanning two centuries demonstrates an increase in maternal mortality rates after child loss on both sides of a major demographic transition.
Conditional gene knockout in combination with fluorescent labeling of different alleles can be achieved with high efficiency in zebrafish through NHEJ-mediated targeted insertion using a CRISPR/Cas system.